Projekte

Proteome Analysis Reveals Distinct Mitochondrial Functions Linked to Interferon Response Patterns in Activated CD4+and CD8+T Cells

Autor(en)
Marlene C. Gerner, Laura Niederstätter, Liesa Ziegler, Andrea Bileck, Astrid Slany, Lukas Janker, Ralf L. J. Schmidt, Christopher Gerner, Giorgia Del Favero, Klaus G. Schmetterer
Abstrakt

While genetic traits and epigenetic modifications mainly encode cell type-specific effector functions, the eventual outcome is also prone to modulation by post-transcriptional regulation mechanisms. T cells are a powerful model for the investigation of such modulatory effects, as common precursor cells may differentiate either to helper CD4(+) T cells or cytotoxic CD8(+) cells, which elicit distinct functionalities upon TCR-stimulation. Human primary CD4(+) and CD8(+) T cells were purified from three individual donors and activated with anti-CD3/CD28 antibodies. Associated proteome alterations were analyzed by high-resolution mass spectrometry using a label-free shotgun approach. Metabolic activation was indicated by upregulation of enzymes related to glycolysis, NADH production, fatty acid synthesis, and uptake as well as amino acid and iron uptake. Besides various inflammatory effector molecules, the mitochondrial proteins CLUH, TFAM, and TOMM34 were found specifically induced in CD4+ T cells. Investigation of overrepresented conserved transcription binding sites by the oPOSSUM software suggested interferon type I inducer IRF1 to cause many of the observed proteome alterations in CD4(+) T cells. RT qPCR demonstrated the specific induction of IRF1 in CD4(+) T cells only. While the interferon regulatory factor IRF4 was found induced in both T cell subtypes at protein and mRNA level, IRF9 and the type I interferon-induced proteins IFIT1, IFIT3, and MX1 were only found induced in CD4(+) T cells. As oxidative stress enhances mitochondrial DNA-dependent type I interferon responses, the present data suggested that mitochondrial activities regulate those cell type-specific signaling pathways. Indeed, we detected mitochondrial superoxide formation predominantly in CD4(+) T cells via FACS analysis with MitoSOX (TM) and confirmed this observation by live cell imaging with confocal microscopy. As interferon signaling regulates important features such as resistance regarding immune checkpoint blockade therapy, the present data may identify potential new targets for the efficient control of highly relevant immune cell properties.

Organisation(en)
Institut für Analytische Chemie, Joint Metabolome Facility, Massenspektrometriezentrum, Institut für Lebensmittelchemie und Toxikologie
Externe Organisation(en)
Medizinische Universität Wien
Journal
Frontiers in Pharmacology
Band
10
Anzahl der Seiten
13
ISSN
1663-9812
DOI
https://doi.org/10.3389/fphar.2019.00727
Publikationsdatum
07-2019
Peer-reviewed
Ja
ÖFOS 2012
Proteomik, Pharmakologie, Analytische Chemie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/proteome-analysis-reveals-distinct-mitochondrial-functions-linked-to-interferon-response-patterns-in-activated-cd4and-cd8t-cells(e88db2c5-c8a0-45df-812e-80e040c513b5).html

Publikationen

Proteome Analysis Reveals Distinct Mitochondrial Functions Linked to Interferon Response Patterns in Activated CD4+and CD8+T Cells

Autor(en)
Marlene C. Gerner, Laura Niederstätter, Liesa Ziegler, Andrea Bileck, Astrid Slany, Lukas Janker, Ralf L. J. Schmidt, Christopher Gerner, Giorgia Del Favero, Klaus G. Schmetterer
Abstrakt

While genetic traits and epigenetic modifications mainly encode cell type-specific effector functions, the eventual outcome is also prone to modulation by post-transcriptional regulation mechanisms. T cells are a powerful model for the investigation of such modulatory effects, as common precursor cells may differentiate either to helper CD4(+) T cells or cytotoxic CD8(+) cells, which elicit distinct functionalities upon TCR-stimulation. Human primary CD4(+) and CD8(+) T cells were purified from three individual donors and activated with anti-CD3/CD28 antibodies. Associated proteome alterations were analyzed by high-resolution mass spectrometry using a label-free shotgun approach. Metabolic activation was indicated by upregulation of enzymes related to glycolysis, NADH production, fatty acid synthesis, and uptake as well as amino acid and iron uptake. Besides various inflammatory effector molecules, the mitochondrial proteins CLUH, TFAM, and TOMM34 were found specifically induced in CD4+ T cells. Investigation of overrepresented conserved transcription binding sites by the oPOSSUM software suggested interferon type I inducer IRF1 to cause many of the observed proteome alterations in CD4(+) T cells. RT qPCR demonstrated the specific induction of IRF1 in CD4(+) T cells only. While the interferon regulatory factor IRF4 was found induced in both T cell subtypes at protein and mRNA level, IRF9 and the type I interferon-induced proteins IFIT1, IFIT3, and MX1 were only found induced in CD4(+) T cells. As oxidative stress enhances mitochondrial DNA-dependent type I interferon responses, the present data suggested that mitochondrial activities regulate those cell type-specific signaling pathways. Indeed, we detected mitochondrial superoxide formation predominantly in CD4(+) T cells via FACS analysis with MitoSOX (TM) and confirmed this observation by live cell imaging with confocal microscopy. As interferon signaling regulates important features such as resistance regarding immune checkpoint blockade therapy, the present data may identify potential new targets for the efficient control of highly relevant immune cell properties.

Organisation(en)
Institut für Analytische Chemie, Joint Metabolome Facility, Massenspektrometriezentrum, Institut für Lebensmittelchemie und Toxikologie
Externe Organisation(en)
Medizinische Universität Wien
Journal
Frontiers in Pharmacology
Band
10
Anzahl der Seiten
13
ISSN
1663-9812
DOI
https://doi.org/10.3389/fphar.2019.00727
Publikationsdatum
07-2019
Peer-reviewed
Ja
ÖFOS 2012
Proteomik, Pharmakologie, Analytische Chemie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/proteome-analysis-reveals-distinct-mitochondrial-functions-linked-to-interferon-response-patterns-in-activated-cd4and-cd8t-cells(e88db2c5-c8a0-45df-812e-80e040c513b5).html

Vortraege

Proteome Analysis Reveals Distinct Mitochondrial Functions Linked to Interferon Response Patterns in Activated CD4+and CD8+T Cells

Autor(en)
Marlene C. Gerner, Laura Niederstätter, Liesa Ziegler, Andrea Bileck, Astrid Slany, Lukas Janker, Ralf L. J. Schmidt, Christopher Gerner, Giorgia Del Favero, Klaus G. Schmetterer
Abstrakt

While genetic traits and epigenetic modifications mainly encode cell type-specific effector functions, the eventual outcome is also prone to modulation by post-transcriptional regulation mechanisms. T cells are a powerful model for the investigation of such modulatory effects, as common precursor cells may differentiate either to helper CD4(+) T cells or cytotoxic CD8(+) cells, which elicit distinct functionalities upon TCR-stimulation. Human primary CD4(+) and CD8(+) T cells were purified from three individual donors and activated with anti-CD3/CD28 antibodies. Associated proteome alterations were analyzed by high-resolution mass spectrometry using a label-free shotgun approach. Metabolic activation was indicated by upregulation of enzymes related to glycolysis, NADH production, fatty acid synthesis, and uptake as well as amino acid and iron uptake. Besides various inflammatory effector molecules, the mitochondrial proteins CLUH, TFAM, and TOMM34 were found specifically induced in CD4+ T cells. Investigation of overrepresented conserved transcription binding sites by the oPOSSUM software suggested interferon type I inducer IRF1 to cause many of the observed proteome alterations in CD4(+) T cells. RT qPCR demonstrated the specific induction of IRF1 in CD4(+) T cells only. While the interferon regulatory factor IRF4 was found induced in both T cell subtypes at protein and mRNA level, IRF9 and the type I interferon-induced proteins IFIT1, IFIT3, and MX1 were only found induced in CD4(+) T cells. As oxidative stress enhances mitochondrial DNA-dependent type I interferon responses, the present data suggested that mitochondrial activities regulate those cell type-specific signaling pathways. Indeed, we detected mitochondrial superoxide formation predominantly in CD4(+) T cells via FACS analysis with MitoSOX (TM) and confirmed this observation by live cell imaging with confocal microscopy. As interferon signaling regulates important features such as resistance regarding immune checkpoint blockade therapy, the present data may identify potential new targets for the efficient control of highly relevant immune cell properties.

Organisation(en)
Institut für Analytische Chemie, Joint Metabolome Facility, Massenspektrometriezentrum, Institut für Lebensmittelchemie und Toxikologie
Externe Organisation(en)
Medizinische Universität Wien
Journal
Frontiers in Pharmacology
Band
10
Anzahl der Seiten
13
ISSN
1663-9812
DOI
https://doi.org/10.3389/fphar.2019.00727
Publikationsdatum
07-2019
Peer-reviewed
Ja
ÖFOS 2012
Proteomik, Pharmakologie, Analytische Chemie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/proteome-analysis-reveals-distinct-mitochondrial-functions-linked-to-interferon-response-patterns-in-activated-cd4and-cd8t-cells(e88db2c5-c8a0-45df-812e-80e040c513b5).html