Projekte

In vitro Radiopharmaceutical Evidence for MCHR1 Binding Sites in Murine Brown Adipocytes

Autor(en)
Theresa Balber, Katarina Bencurova, Florian Wolfgang Kiefer, Oana Cristina Kulterer, Eva-Maria Klebermass, Gerda Egger, Loan Tran, Karl-Heinz Wagner, Helmut Viernstein, Katharina Pallitsch, Helmut Spreitzer, Marcus Hacker, Wolfgang Wadsak, Markus Mitterhauser, Cecile Philippe
Abstrakt

[C-11]SNAP-7941 and its radiofluorinated, fluoro-ethyl derivative [F-18]FE@SNAP have been developed as the first positron emission tomography tracers for melanin-concentrating hormone receptor 1 (MCHR1) imaging. Accumulation of these MCHR1 PET-tracers in rat brown adipose tissue (BAT) in vivo provided first indication of MCHR1 expression in rodent BAT. To rule out off-target binding, affinity of both MCHR1 ligands toward adrenergic beta-3 receptors (ADRB3) was examined. Further, specific binding of [C-11]SNAP-7941 to brown adipocytes and effects of MCHR1 ligands on brown adipocyte activation were investigated. SNAP-7941 and FE@SNAP evinced to be highly selective toward MCHR1. [C-11]SNAP-7941 binding to brown adipocytes was shown to be mainly MCHR1-specific. This data strongly indicates MCHR1 expression in rodent BAT and moreover, a peripheral, anti-obesity effect of MCHR1 antagonists directly exerted in BAT is proposed. Moreover, MCHR1 expression in murine brown adipocytes was confirmed by protein and mRNA analysis. We conclude that MCHR1 PET imaging contributes to basic research in endocrinology by elucidating the involvement of the MCH system in peripheral tissues, such as BAT.

Organisation(en)
Department für Pharmazeutische Technologie und Biopharmazie, Forschungsplattform Active Ageing, Department für Ernährungswissenschaften, Institut für Organische Chemie, Department für Pharmazeutische Chemie
Externe Organisation(en)
Medizinische Universität Wien, Centre for Biomarker Research in Medicine – CBmed GmbH
Journal
Frontiers in Endocrinology
Band
10
Anzahl der Seiten
10
ISSN
1664-2392
DOI
https://doi.org/10.3389/fendo.2019.00324
Publikationsdatum
06-2019
Peer-reviewed
Ja
ÖFOS 2012
Endokrinologie, Pharmazeutische Technologie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/in-vitro-radiopharmaceutical-evidence-for-mchr1-binding-sites-in-murine-brown-adipocytes(e0419bd6-a69a-496f-8e08-7e659b84f098).html

Publikationen

In vitro Radiopharmaceutical Evidence for MCHR1 Binding Sites in Murine Brown Adipocytes

Autor(en)
Theresa Balber, Katarina Bencurova, Florian Wolfgang Kiefer, Oana Cristina Kulterer, Eva-Maria Klebermass, Gerda Egger, Loan Tran, Karl-Heinz Wagner, Helmut Viernstein, Katharina Pallitsch, Helmut Spreitzer, Marcus Hacker, Wolfgang Wadsak, Markus Mitterhauser, Cecile Philippe
Abstrakt

[C-11]SNAP-7941 and its radiofluorinated, fluoro-ethyl derivative [F-18]FE@SNAP have been developed as the first positron emission tomography tracers for melanin-concentrating hormone receptor 1 (MCHR1) imaging. Accumulation of these MCHR1 PET-tracers in rat brown adipose tissue (BAT) in vivo provided first indication of MCHR1 expression in rodent BAT. To rule out off-target binding, affinity of both MCHR1 ligands toward adrenergic beta-3 receptors (ADRB3) was examined. Further, specific binding of [C-11]SNAP-7941 to brown adipocytes and effects of MCHR1 ligands on brown adipocyte activation were investigated. SNAP-7941 and FE@SNAP evinced to be highly selective toward MCHR1. [C-11]SNAP-7941 binding to brown adipocytes was shown to be mainly MCHR1-specific. This data strongly indicates MCHR1 expression in rodent BAT and moreover, a peripheral, anti-obesity effect of MCHR1 antagonists directly exerted in BAT is proposed. Moreover, MCHR1 expression in murine brown adipocytes was confirmed by protein and mRNA analysis. We conclude that MCHR1 PET imaging contributes to basic research in endocrinology by elucidating the involvement of the MCH system in peripheral tissues, such as BAT.

Organisation(en)
Department für Pharmazeutische Technologie und Biopharmazie, Forschungsplattform Active Ageing, Department für Ernährungswissenschaften, Institut für Organische Chemie, Department für Pharmazeutische Chemie
Externe Organisation(en)
Medizinische Universität Wien, Centre for Biomarker Research in Medicine – CBmed GmbH
Journal
Frontiers in Endocrinology
Band
10
Anzahl der Seiten
10
ISSN
1664-2392
DOI
https://doi.org/10.3389/fendo.2019.00324
Publikationsdatum
06-2019
Peer-reviewed
Ja
ÖFOS 2012
Endokrinologie, Pharmazeutische Technologie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/in-vitro-radiopharmaceutical-evidence-for-mchr1-binding-sites-in-murine-brown-adipocytes(e0419bd6-a69a-496f-8e08-7e659b84f098).html

Vortraege

In vitro Radiopharmaceutical Evidence for MCHR1 Binding Sites in Murine Brown Adipocytes

Autor(en)
Theresa Balber, Katarina Bencurova, Florian Wolfgang Kiefer, Oana Cristina Kulterer, Eva-Maria Klebermass, Gerda Egger, Loan Tran, Karl-Heinz Wagner, Helmut Viernstein, Katharina Pallitsch, Helmut Spreitzer, Marcus Hacker, Wolfgang Wadsak, Markus Mitterhauser, Cecile Philippe
Abstrakt

[C-11]SNAP-7941 and its radiofluorinated, fluoro-ethyl derivative [F-18]FE@SNAP have been developed as the first positron emission tomography tracers for melanin-concentrating hormone receptor 1 (MCHR1) imaging. Accumulation of these MCHR1 PET-tracers in rat brown adipose tissue (BAT) in vivo provided first indication of MCHR1 expression in rodent BAT. To rule out off-target binding, affinity of both MCHR1 ligands toward adrenergic beta-3 receptors (ADRB3) was examined. Further, specific binding of [C-11]SNAP-7941 to brown adipocytes and effects of MCHR1 ligands on brown adipocyte activation were investigated. SNAP-7941 and FE@SNAP evinced to be highly selective toward MCHR1. [C-11]SNAP-7941 binding to brown adipocytes was shown to be mainly MCHR1-specific. This data strongly indicates MCHR1 expression in rodent BAT and moreover, a peripheral, anti-obesity effect of MCHR1 antagonists directly exerted in BAT is proposed. Moreover, MCHR1 expression in murine brown adipocytes was confirmed by protein and mRNA analysis. We conclude that MCHR1 PET imaging contributes to basic research in endocrinology by elucidating the involvement of the MCH system in peripheral tissues, such as BAT.

Organisation(en)
Department für Pharmazeutische Technologie und Biopharmazie, Forschungsplattform Active Ageing, Department für Ernährungswissenschaften, Institut für Organische Chemie, Department für Pharmazeutische Chemie
Externe Organisation(en)
Medizinische Universität Wien, Centre for Biomarker Research in Medicine – CBmed GmbH
Journal
Frontiers in Endocrinology
Band
10
Anzahl der Seiten
10
ISSN
1664-2392
DOI
https://doi.org/10.3389/fendo.2019.00324
Publikationsdatum
06-2019
Peer-reviewed
Ja
ÖFOS 2012
Endokrinologie, Pharmazeutische Technologie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/in-vitro-radiopharmaceutical-evidence-for-mchr1-binding-sites-in-murine-brown-adipocytes(e0419bd6-a69a-496f-8e08-7e659b84f098).html