Projekte
Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus
- Autor(en)
- Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
- Abstrakt
Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.
- Organisation(en)
- Institut für Lebensmittelchemie und Toxikologie, Institut für Biologische Chemie, Department für Mikrobiologie und Ökosystemforschung
- Externe Organisation(en)
- Karlsruher Institut für Technologie, Université de Montpellier
- Journal
- Chemical Communications
- Band
- 55
- Seiten
- 9649-9652
- Anzahl der Seiten
- 4
- ISSN
- 1359-7345
- DOI
- https://doi.org/10.1039/c9cc02215e
- Publikationsdatum
- 08-2019
- Peer-reviewed
- Ja
- ÖFOS 2012
- 104004 Chemische Biologie
- Link zum Portal
- https://ucris.univie.ac.at/portal/de/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html
Publikationen
Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus
- Autor(en)
- Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
- Abstrakt
Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.
- Organisation(en)
- Institut für Lebensmittelchemie und Toxikologie, Institut für Biologische Chemie, Department für Mikrobiologie und Ökosystemforschung
- Externe Organisation(en)
- Karlsruher Institut für Technologie, Université de Montpellier
- Journal
- Chemical Communications
- Band
- 55
- Seiten
- 9649-9652
- Anzahl der Seiten
- 4
- ISSN
- 1359-7345
- DOI
- https://doi.org/10.1039/c9cc02215e
- Publikationsdatum
- 08-2019
- Peer-reviewed
- Ja
- ÖFOS 2012
- 104004 Chemische Biologie
- Link zum Portal
- https://ucris.univie.ac.at/portal/de/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html
Vortraege
Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus
- Autor(en)
- Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
- Abstrakt
Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.
- Organisation(en)
- Institut für Lebensmittelchemie und Toxikologie, Institut für Biologische Chemie, Department für Mikrobiologie und Ökosystemforschung
- Externe Organisation(en)
- Karlsruher Institut für Technologie, Université de Montpellier
- Journal
- Chemical Communications
- Band
- 55
- Seiten
- 9649-9652
- Anzahl der Seiten
- 4
- ISSN
- 1359-7345
- DOI
- https://doi.org/10.1039/c9cc02215e
- Publikationsdatum
- 08-2019
- Peer-reviewed
- Ja
- ÖFOS 2012
- 104004 Chemische Biologie
- Link zum Portal
- https://ucris.univie.ac.at/portal/de/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html