Projekte

Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

Autor(en)
Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
Abstrakt

Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.

Organisation(en)
Institut für Lebensmittelchemie und Toxikologie, Institut für Biologische Chemie, Department für Mikrobiologie und Ökosystemforschung
Externe Organisation(en)
Karlsruher Institut für Technologie, Université de Montpellier
Journal
Chemical Communications
Band
55
Seiten
9649-9652
Anzahl der Seiten
4
ISSN
1359-7345
DOI
https://doi.org/10.1039/c9cc02215e
Publikationsdatum
08-2019
Peer-reviewed
Ja
ÖFOS 2012
Chemische Biologie
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html

Publikationen

Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

Autor(en)
Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
Abstrakt

Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.

Organisation(en)
Institut für Lebensmittelchemie und Toxikologie, Institut für Biologische Chemie, Department für Mikrobiologie und Ökosystemforschung
Externe Organisation(en)
Karlsruher Institut für Technologie, Université de Montpellier
Journal
Chemical Communications
Band
55
Seiten
9649-9652
Anzahl der Seiten
4
ISSN
1359-7345
DOI
https://doi.org/10.1039/c9cc02215e
Publikationsdatum
08-2019
Peer-reviewed
Ja
ÖFOS 2012
Chemische Biologie
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html

Vortraege

Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

Autor(en)
Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
Abstrakt

Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.

Organisation(en)
Institut für Lebensmittelchemie und Toxikologie, Institut für Biologische Chemie, Department für Mikrobiologie und Ökosystemforschung
Externe Organisation(en)
Karlsruher Institut für Technologie, Université de Montpellier
Journal
Chemical Communications
Band
55
Seiten
9649-9652
Anzahl der Seiten
4
ISSN
1359-7345
DOI
https://doi.org/10.1039/c9cc02215e
Publikationsdatum
08-2019
Peer-reviewed
Ja
ÖFOS 2012
Chemische Biologie
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html