Projects

Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

Author(s)
Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
Abstract

Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.

Organisation(s)
Department of Food Chemistry and Toxicology, Department of Biological Chemistry, Department of Microbiology and Ecosystem Science
External organisation(s)
Karlsruher Institut für Technologie, Université de Montpellier
Journal
Chemical Communications
Volume
55
Pages
9649-9652
No. of pages
4
ISSN
1359-7345
DOI
https://doi.org/10.1039/c9cc02215e
Publication date
08-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
104004 Chemical biology
Portal url
https://ucris.univie.ac.at/portal/en/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html

Publications

Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

Author(s)
Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
Abstract

Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.

Organisation(s)
Department of Food Chemistry and Toxicology, Department of Biological Chemistry, Department of Microbiology and Ecosystem Science
External organisation(s)
Karlsruher Institut für Technologie, Université de Montpellier
Journal
Chemical Communications
Volume
55
Pages
9649-9652
No. of pages
4
ISSN
1359-7345
DOI
https://doi.org/10.1039/c9cc02215e
Publication date
08-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
104004 Chemical biology
Portal url
https://ucris.univie.ac.at/portal/en/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html

Talks

Silica particles with a quercetin-R5 peptide conjugate are taken up into HT-29 cells and translocate into the nucleus

Author(s)
Giorgia Del Favero, Friedrich Bialas, Stephanie Grabher-Wusche, Anja Wittig, Birgit Bräuer, Dagmar Gerthsen, Cecile Echalier, Meder Kamalov, Doris Marko, Christian F. W. Becker
Abstract

Intracellular delivery of bioactive polyphenols is currently evaluated as a protective strategy for cells under pharmaceutical stress. To this end, the 20mer R5 peptide from the marine diatom C. fusiformis was N-terminally modified with a quercetin derivative. This polyphenol-peptide conjugate was used to generate homogeneous silica particles under biomimetic conditions that are efficiently taken up by eukaryotic cells without being cytotoxic. However, not only was accumulation in the cytoplasm of living cells observed via electron and fluorescence microscopy but also translocation into the nucleus. The latter was only seen when the quercetin-peptide conjugate was present within the silica particles and provides a novel targeting option for silica particles to nuclei.

Organisation(s)
Department of Food Chemistry and Toxicology, Department of Biological Chemistry, Department of Microbiology and Ecosystem Science
External organisation(s)
Karlsruher Institut für Technologie, Université de Montpellier
Journal
Chemical Communications
Volume
55
Pages
9649-9652
No. of pages
4
ISSN
1359-7345
DOI
https://doi.org/10.1039/c9cc02215e
Publication date
08-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
104004 Chemical biology
Portal url
https://ucris.univie.ac.at/portal/en/publications/silica-particles-with-a-quercetinr5-peptide-conjugate-are-taken-up-into-ht29-cells-and-translocate-into-the-nucleus(dbd55566-87f1-404c-933d-84d3a73d7523).html